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KMID : 0861020130280020033
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Korea Journal of Herbology
2013 Volume.28 No. 2 p.33 ~ p.38
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Effects of Liriopis Tuber on 4-HNE-induced Apoptosis in PC-12 Cells
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박수환:Park Soo-Hwan
김연섭:Kim Youn-Sub
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Abstract
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Objects : Apoptosis leads to the death of a cell. The mitochondrial pathway of apoptosis, a process regulated by
the Bcl-2 family of proteins, plays a key role in various biological processes. The tuber of Liriope platyphylla
Wang et TANG (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic,
antitussive, expectorant and anti-asthmatic properties. The purpose of this study was to observe the effect of the
Liriopis tuber on mitochondrial -mediated apoptosis in PC-12 cells.
Method : A cytotoxic test on Liriopis Tuber water extract was conducted and another MTT assay was conducted
to observe the cytoprotective effect against 4-HNE 25 μM that causes oxidative stress in PC-12 cells for 24
hours. In addition, in order to observe the expression of Bcl-2 and Bax protein involved with apoptosis, western
blot was conducted.
Results : The LT water extract had no toxicity for PC-12 cell. In the cytoprotective effect against 4-HNE,
both of the group treated with 50 μg/㎖ and 100 μg/㎖ of LT water extract showed a significant increase in
comparison with the control group. In Bax protein expression, all the experimental groups treated with LT
water extract showed a decrease in comparison with the control group but had no significance. In Bcl-2
protein expression, all the experimental groups treated with LT water extract showed a significant increase in
comparison with the control group.
Conclusion : These results suggest that LT is effective in reducing apoptosis.
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KEYWORD
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Liriopis Tuber, 4-HNE, MTT assay, Bax, Bcl-2
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